Determination of triiodothyronine concentration in human serum
نویسندگان
چکیده
منابع مشابه
Serum triiodothyronine determination in clinical use.
Two radioimmunassays for the determination of serum triiodothyronine (T3) were developed. The assay of T3 in unextracted serum had several advantages over the assay on extracted serum and was chosen for the routine determination of T3 in serum from 117 patients requiring assessment of their thyroid status. In 53 subjects considered retrospectively not to have thyroid dysfunction nor to have bee...
متن کاملDirect immunoassay of triiodothyronine in human serum.
A sensitive and precise radioimmunoassay for the direct measurement of triiodothyronine (T(3)) in human serum has been designed using sodium salicylate to block T(3)-TBG binding. This assay is sufficiently sensitive to quantitate T(3) accurately in 50-100 mul of normal serum and to measure quantities as small as 12.5 pg in 0.2 ml of hypothyroid serum. The T(3) values observed in euthyroid subje...
متن کاملRadioimmunoassay of triiodothyronine in unextracted human serum.
Serum triiodothyronine (T-3) concentrations have been estimated by radioimmunoassay using unextracted serum. The serum T-3 concentrations have been shown to be similar in two separate European populations (0.76-1.67 ng/ml). Raised T-3 values have been observed in all subjects with hyperthyroidism. Low values are seen in hypothyroidism although there is some overlap with the normal range. There ...
متن کاملTotal and free triiodothyronine in human serum.
A reliable method has been developed for the determination of total serum T3, dialyzable fraction (DFT3), and absolute concentration of free T3 (AFT3). Total T3 values (mean +/- SD) were: healthy euthyroid subjects, 0.33 +/- 0.07 mug per 100 ml; hyperthyroid patients, 0.71 +/- 0.1 mug per 100 ml; hypothyroid, 0.10 +/- 0.03 mug per 100 ml. Values (mean +/- SD) for DFT3 in these groups were 0.46 ...
متن کاملHIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF THEOPHYLLINE IN HUMAN SERUM
A simple, rapid and sensitive HPLC method for the determination of theophylline (T) in human serum has been developed. An isocratic system consisting of a /-l Bondapak C18 column, mobile phase of methanol, phosphate buffer (22:78, pH=4.5), and a flow rate of 1.4 mL/min was used. The eluent was detected by UV at 275 nm at room temperature. 8-Chlorotheophylline (8-CT) was used as an internal ...
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ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1969
ISSN: 0021-9738
DOI: 10.1172/jci106072